The Virgin and Child with St Anne and St John the Baptist, sometimes called The Burlington House Cartoon, is a drawing by Leonardo da Vinci, believed to have been executed c.1499-1500. The drawing is incharcoal and black and white chalk, on eight sheets of paper glued together. Because of its large size and format the drawing is presumed to be a cartoon for a painting. No painting by Leonardo exists that is based directly on this cartoon.

The drawing depicts the Virgin Mary seated on the knees of her mother St Anne and holding the Child Jesus while St. John the Baptist, the cousin of Jesus, stands to the right. It currently hangs in the National Gallery in London.